Development of efficient method of generating reactive oxygen species by expanding cavitation region using ultrasound focus scanning in the direction of ultrasound propagation

Abstract Sonodynamic therapy is a minimally invasive high-intensity focused ultrasound (HIFU) based that combines sonosensitizer and reactive oxygen species (ROS), which are produced by acoustic cavitation. In this paper, we experimentally examined the effectiveness of proposed method scanning focus in direction HIFU propagation for continuously generating cavitation clouds to expand region ROS generation increase amount ROS. First, continuous bubbles was observed using high-speed camera, it confirmed could be generated even with short-duration HIFU, difficult generate itself. Subsequently, evaluated area imaging sonochemiluminescence. Comparing irradiation at 1 point 7 points, per energy increased about 1.5 times, suggesting an efficient generation.